Abstract
A flow-injection method with photochemically induced fluorescence (PF) or native fluorescence (NF) detection was developed for the determination of four sulphonamides. Sulphamethazine was determined using PF and sulphanilamide, sulphaguanidine and sulphacetamide using NF. Linear calibration graphs were obtained over a concentration range of one order of magnitude. The relative standard deviations were within the range 0.4–5%. Limits of detection were between 0.015 and 0.12 μg ml −1. The method was applied to the determination of sulphonamides in milk and pharmaceutical preparations.
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