Abstract
The significant healing effect of Tilia platyphyllos Scop. and Tilia cordata Mill. flowers are well known. However, the flowers of Tilia tomentosa Moench. are not suitable for harvest due to their toxic effects. To investigate the diverse background of this effect, we applied a functional miRNA-based marker, mannose expression analysis and determined the content of bioactive compounds. Out of nine tested markers, three (miR160, miR167 and miR408) provided reproducible miRNA-based loci and two of them (miR160 and miR398) enabled the acquisition of fingerprinting specific to flower and leaf samples of T. platyphyllos and T. tomentosa. The most pronounced profiling was specific for miR408 marker, the function of which is connected to plant defense and adaptation mechanisms. We confirmed the suitability of microRNA-based markers for polymorphism determination of flowers of selected species of lime-tree. The highest values of antioxidant activity, flavonoids, total polyphenols and phenolic acids content have been reached in silver linden flowers. When comparing the transcription activity of mannose in flowers, more than 30 times higher levels of mannose transcripts for the silver linden flowers was observed.
Highlights
IntroductionTo investigate the diverse background of this effect, we applied a functional miRNAbased marker, mannose expression analysis and determined the content of bioactive compounds
The most amplification by the marker miR160Inwas the were amplified by markers based on miR408, where the most (48%) miRNA loci were flower sample of the small-leaved linden (T. cordata), leaf sample of large-leaved linden detected in flower samples, 30% in leaf samples and 22% in bracts samples
The results indicate that the highest content of total flavonoids, total polyphenols and phenolic acids is presented in flowers of silver lime (T. tomentosa)
Summary
To investigate the diverse background of this effect, we applied a functional miRNAbased marker, mannose expression analysis and determined the content of bioactive compounds. Out of nine tested markers, three (miR160, miR167 and miR408) provided reproducible miRNA-based loci and two of them (miR160 and miR398) enabled the acquisition of fingerprinting specific to flower and leaf samples of T. platyphyllos and T. tomentosa. We confirmed the suitability of microRNA-based markers for polymorphism determination of flowers of selected species of lime-tree. The species of lime-tree (Tilia spp.) from the Malvaceae family are beautiful, stout trees with a dense, shady crown. They grow to a height of 30 m and can live up to 700 years
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