Abstract
In this study, the catalytic activity and stability of flowerlike hybrid horseradish peroxidase (HRP) nanobiocatalyst (HRP-Cu 2+ ) obtained from Cu 2+ ions and HRP enzyme in the polymerization reaction of guaiacol were analyzed. We demonstrated that HRP-Cu 2+ and hydrogen peroxide (H 2 O 2 ) initiator showed significantly increased catalytic activity and stability on the polymerization of guaiacol compared to that of free HRP enzyme. Poly(guaiacol) was observed with quite high yields (88%) and molecular weights (38,000 g/mol) under pH 7.4 phosphate-buffered saline (PBS) conditions at 60 °C with 5 weight% of HRP-Cu 2+ loading. HRP-Cu 2+ also shows very high thermal stability and works even at 70 °C reaction temperature; free HRP enzyme denatures at that temperature. Furthermore, HRP-Cu 2+ provided considerable repeated use and showed some degree of catalytic activity, even after the fourth recycle, in the polymerization of guaiacol.
Highlights
Enzyme biocatalysts are used in many scientific and technological fields such as chemistry, biochemistry, and medicine because of their high catalytic activity, selectivity, low toxicity, and water-soluble properties [1,2,3,4,5,6,7]
Aniline and phenol derivatives can be successfully polymerized in the presence of horseradish peroxidase (HRP) enzyme and H2O2
The coordination between Cu2+ ions and amide nitrogen atoms in the protein structure of HRP enzyme formed complexes is the principal step for the formation of hybrid nanoflowers (HRP-Cu2+) [27]
Summary
Enzyme biocatalysts are used in many scientific and technological fields such as chemistry, biochemistry, and medicine because of their high catalytic activity, selectivity, low toxicity, and water-soluble properties [1,2,3,4,5,6,7]. A variety of reactions can be catalyzed by enzymes under milder conditions and enzymes indicate activity against certain substrates or functional groups. Peroxidase enzymes achieve oxidation reactions of proton donor compounds with H2O2 oxidizer/. Peroxidase enzymes, such as horseradish peroxidase (HRP), contain iron (Fe) ions in their active centers [8]. These enzymes are used for radicalic reactions/polymerizations. Aniline and phenol derivatives can be successfully polymerized in the presence of HRP enzyme and H2O2. Peroxidase enzymes can oxidize different electron donor compounds including indoles, phenolic acids, and sulfates [9,10,11,12,13,14,15,16]
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