Flower-shaped gold nanoparticles synthesized using Kedrostis foetidissima and their antiproliferative activity against bone cancer cell lines

M Jannathul Firdhouse,P Lalitha

doi:10.1007/s40090-016-0098-4

Abstract

Three different methods were employed for the synthesis of biogenic gold nanoparticles using the aqueous extracts of Kedrostis foetidissima. The interaction of gold nanoparticles with the phytoconstituents was investigated by FTIR. The complete reduction of chloroaurate ions to gold nanoparticles was monitored using UV–visible spectroscopy under the different plant extract concentration and conditions. The formation of gold nanoparticles was confirmed using XRD, SEM and TEM analysis. The anisotropic and flower-shaped gold nanoparticles of size below 25 nm was confirmed by TEM analysis. Cucurbitacins, the chief constituents of K. foetidissima probably might have interacted with the chloroaurate ions, facilitating the formation of gold nanoparticles. KFL-mediated AuNPs showed 88 % cell viability against bone cancer cell lines at 200 µg/ml concentration using MTT assay. This may be due to the formation of flower-shaped nature of KFL-mediated AuNPs. The novelty of the work lies in the plant-mediated synthesis of biocompatible gold nanoparticles of size less than 25 nm showing 88 % of cell viability against bone cancer cell lines.

Highlights

In modern science, one of the most dynamic areas of research is found to be in nanotechnology field which creates a force towards human life [1]
Nanochemistry mainly focuses on the synthesis of various metal nanoparticles like gold, silver, zinc, platinum [2,3,4,5], etc., due to its unique properties, which opens a new venue for the production of nanodevices, therapeutics, drugs, etc., in the field of nanomedicine and nanobiotechnology [6,7,8,9,10,11,12]
The aqueous extracts of KFL and KFS was tested for the reduction of chloroaurate ions to gold nanoparticles

Summary

Introduction

One of the most dynamic areas of research is found to be in nanotechnology field which creates a force towards human life [1]. Nanochemistry mainly focuses on the synthesis of various metal nanoparticles like gold, silver, zinc, platinum [2,3,4,5], etc., due to its unique properties, which opens a new venue for the production of nanodevices, therapeutics, drugs, etc., in the field of nanomedicine and nanobiotechnology [6,7,8,9,10,11,12]. Gold nanoparticles have diverse applications in cancer treatment, electrochemical sensors, biosensors, gene and drug delivery, catalysis, etc., [13, 14]. The superior properties of the metal nanoparticles depend upon the size, shape and dispersed nature which is different from that of the bulk materials. Nanoparticles can be prepared by physical and chemical methods. The eco-friendly synthetic procedures for nanoparticles have been adopted by researchers [15, 16]

Methods

Results

Conclusion