Abstract
AbstractThis work aims to characterize the mixing and suspension dynamics occurring within two commercially available DASGIP bioreactor configurations, equipped with a two‐blade paddle impeller with large impeller to tank diameter ratio, D/T = 0.97. Both continuous and intermittent agitation modes were employed to determine the impact that agitation strategy has upon mass transfer and microcarrier settling/suspension. This paper builds upon the flow dynamics data presented in Part 1 for a flat bottom DASGIP bioreactor and shows how intermittent agitation can break‐up regions of slow mixing observed during continuous agitation, therefore substantially increasing the mixing efficiency of the system. Similarly, it was found that microcarrier characteristics might significantly affect the level of suspension when the impeller is in dwell status when intermittent agitation modes are used.
Highlights
Characterization of the flow dynamics in stirred tanks of standard geometry is well documented in the literature
The following section is divided into four parts; Sections 3.1 and 3.2 explore the ensemble-averaged and phase-averaged flow characteristics of the flat and round bottom DASGIP configurations, respectively, under continuous agitation modes
The first part of this study aimed at characterizing the flow field for two DASGIP bioreactor configurations operating under continuous agitation
Summary
Characterization of the flow dynamics in stirred tanks of standard geometry is well documented in the literature. The BioBLU (Eppendorf) single use reactor, which is recommended for cell culture applications,[4] has a flat bottom geometry and is equipped with one or two downpumping pitched blade turbine impellers (D/T = 0.42, C/T = 0.25).[5] The DASGIP bioreactors (Eppendorf) are another example,[6] available with both flat and round bottom configurations and equipped with either a pitched blade turbine impeller (D/T = 0.48) or a two-blade paddle impeller of large impeller to tank diameter ratio (D/T = 0.97) Factors such as the impeller design, that is, axial or radial flow types, off-bottom clearance, blade diameter, pitch and thickness, and vessel configuration, that is, geometry, baffled or unbaffled, round or flat bottom, have all been demonstrated to influence the mean flow, turbulence levels and the trailing vortices generated at the back of the blade.[7,8] Distinct mean flow circulation patterns and blade trailing vortices are created from different impeller types.
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