Abstract
The cell size and DNA and protein content of bacteroids fractionated from alfalfa and soybean nodules by buoyant density sedimentation were examined by laser flow-microfluorometry. In alfalfa nodules, these parameters were inversely related to bacteroid density. The bacteroids which sedimented at the highest density ( p = 1.268) resembled free-living Rhizobium meliloti in cell size and protein content but averaged slightly higher in DNA content. The bacteroids which sedimented at the lowest density ( p = 1.224) averaged larger in size and contained significantly more DNA and protein then free-living R. meliloti. Despite the difference in density, bacteroid fractions from soybean nodules and free-living Rhizobium japonicum were similar in all measured parameters.
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