Abstract
A simple, sensitive and fast continuous flow injection system with spectrophotometric detection was proposed for the determination of salbutamol sulphate (SAL) in pharmaceutical formulations using a solid-phase reactor containing PbO2 immobilized in a polymeric matrix. The method was based on oxidation of the reagent (N, N-dimethyl-p-phenelynediamine) with PbO2 producing dimethylbenzoquinone diimine, which is then coupled with SAL in alkaline medium. The blue colored product was monitored at 640 nm. The calibration graph was linear over the range 0.5 to 60 µg mL -1 with a relative standard deviation less than 3.8% (n=33) and sample throughput 60 samples per hour. Solid-phase reactors can be applied to the determination of SAL in a flow injection system with high sensitivity and significant advantages over conventional procedures. Keyword: Reverse-flow injection, Salbutamol sulphate, spectrophotometric.
Highlights
IntroductionSalbutamol sulphate (SAL), chemically known as bis[(1RS)‐2‐[(1,1‐dimethylethyl) amino]‐1
Salbutamol sulphate (SAL), chemically known as bis[(1RS)‐2‐[(1,1‐dimethylethyl) amino]‐1‐[4‐hydroxy‐3‐(hydroxymethyl) phenyl]ethanol]sulphate, is an agonist for β2 receptor which are present in the bronchioles of lungs of human body and acts as a bronchodilator and its cardiovascular effects are less than its bronchodilator actions[1]
To a flask containing 50 μg mL-1 of SAL, a volume of 2 mL of 4 mM of DMPD, 2 mL of 0.5M of ammonium hydroxide and an amount of 0.03 g of PbO2 immobilized on Cellulose acetate (CA) were added
Summary
Salbutamol sulphate (SAL), chemically known as bis[(1RS)‐2‐[(1,1‐dimethylethyl) amino]‐1. In the present work a solid-phase reactor coupled with flow injection system is adopted for estimation of SAL. This strategy has many advantages such as possibility to use reagents that are not available in soluble form and subsequently avoid the time consuming steps for preparation of reagent solutions. N,N-dimethyl-p-phenelynediamine (DMPD) was adopted as a basis to develop a reverse flow injection (rFIA) method using solid phase reactor containing PbO2 as oxidizing agent. The reagent (DMPD) was injected into the stream of SAL solution through the injection valve which oxidized through the solid phase reactor (8 cm, with particles size of 1.18 mm), combined at Y-link with stream of ammonium hydroxide and mixed in RC.
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