Abstract
A new approach for the determination of ethanol in beverages is presented. The hydrogen peroxide generated in the enzymatically catalyzed oxidation of ethanol is measured by the luminol chemiluminescence reaction using potassium hexacyanoferrate(III) as a catalyst. Alcohol oxidase is immobilized on aminopropyl glass beads packed in a glass column. The chemiluminescence measurement is made using an optical fiber to transport the luminescence from the flow cell to the detector in conjunction with a flow system. This assay system responds linearly in the ethanol concentration range 3 × 10 −6-7.5 × 10 −4 M with a precision of 2.4% and an analysis time of 1.5 min.
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