Abstract
In this paper, a fast method for the study on the interactions of a series of drugs used in the treatment of hypertension with human serum albumin (HSA) by flow injection-capillary electrophoresis (FI-CE) was developed based on the principle of frontal analysis (FA). The binding parameters were determined by FI-CE-FA from Scatchard equation and compared with results obtained by non-CE methods and literature values. A multiple linear regression (MLR) model between the drug–protein binding constants ( K) and structural descriptors of drugs was constructed. L-tryptophan ( L-try) and phenylbutazone (PB) were used as displacement reagents to investigate the binding sites of a series of drugs on HSA. The binding synergism effect between drugs and the effects of many metal ions existing in human plasma on protein binding were also investigated systematically.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.