Abstract
A flow injection analysis (FIA) system combined with Rayleigh light scattering (RLS) detection is developed for the sensitive and rapid determination of protein concentration in human serum sample. This method is based on the weak intensity of RLS of Eriochrome Black T (EBT, 2-hydroxy-1-(1-hydroxy-2-naphthylazo)-6-nitronaphthalene-4-sulfonic acid sodium salt), which can be enhanced by the addition of protein in weakly acidic solution. The effects of pH and interfering species on the determination of protein were examined. Calibrations for protein, based on RLS intensity, were linear in the concentration ranges of 7–36 μg/ml for human serum album (HSA) and 8–44 μg/ml for bovine serum album (BSA). The detection limits of the method were found to be 0.882 and 2.507 μg/ml for HSA and BSA, respectively. A relative standard deviation of 0.76% ( n = 5) was obtained with 20 μg/ml HSA standard solution. The FIA–RLS method was more stable than the general RLS method, and the average RSD value of FIA–RLS was less than that of the general RLS. The sample rate was determined to be 90 samples per hour.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
