Abstract
A flow injection method for the quantitative analysis of vancomycin hydrochloride, C66H75Cl2N9O24.HCl (HVCM), based on the reaction with copper (II) ions, is presented. HVCM forms a lilac-blue complex with copper ions at pH≅4.5 in aqueous solutions, with maximum absorption at 555 nm. The detection limit was estimated to be about 8.5×10-5 mol L-1; the quantitation limit is about 2.5×10-4 mol L-1 and about 30 determinations can be performed in an hour. The accuracy of the method was tested through recovery procedures in presence of four different excipients, in the proportion 1:1 w/w. The results were compared with those obtained with the batch spectrophotometric and with the HPLC methods. Statistical comparison was done using the Student’s procedure. Complete agreement was found at a 0.95 significance level between the proposed flow injection and the batch spectrophotometric methods, which present similar precision (RSD: 2.1 % vs. 1.9%).
Highlights
Vancomycin is a glycopeptidic antibiotic very efficient against a number of gram positive microorganisms, inhibiting the synthesis of the bacterial wall [1,2]
With the appearance of resistant microorganisms in the years 1980, and with the improvement of the processes of preparing and purifying, vancomycin acquired again an important role in the combat to the systemic staphylococcic infections in allergic patients to penicillin and in infections caused by staphylococci resistant to methicillin
The method is based on the measurement of the absorbance of the complex formed between vancomycin and copper (II) ions
Summary
Vancomycin is a glycopeptidic antibiotic very efficient against a number of gram positive microorganisms, inhibiting the synthesis of the bacterial wall [1,2]. With the appearance of resistant microorganisms in the years 1980, and with the improvement of the processes of preparing and purifying, vancomycin acquired again an important role in the combat to the systemic staphylococcic infections in allergic patients to penicillin and in infections caused by staphylococci resistant to methicillin. It presents efficacy, administrated orally, to the combat of Clostridium difficile, responsible for colitis that resulted from the use of antibiotics [1,2,7,8]
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