Abstract
Immobilized lactate dehydrogenase (LDH) is used for determination of L-lactate in a continuous flow system. The LDH is immobilized by reaction with glutaraldehyde onto the surface of alkylamino-bonded silica gel and packed into a column in the flow system. The reduction of NAD + occurs simultaneously, and the NADH formed is detected amperometrically. The peak current is linearly related to the L-lactate concentration in the range 1–80 × 10 -6 M; 30 samples h -1 can be analyzed with a r.s.d. of 0.5–1.5%. The immobilized LDH retains over 90% of its initial activity after repetitive use for 3 months.
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