Abstract
The fractionation of high-molecular-mass polyacrylamide in aqueous solutions by size-exclusion chromatography is complicated by concerns over adsorption and shear degradation. The coupling of flow field-flow fractionation (flow FFF), multi-angle laser light scattering (MALLS) detection and a concentration-sensitive detector provided absolute measurement of molecular mass distributions in a low shear environment. Critical to the success of the method was the careful selection of the carrier solution. A number of commercial polyacrylamide standards with mean molecular masses in the range 0.35 to 9.00·10 6 were studied. The breadth of the distributions indicated the presence of entangled or agglomerated polymer. Diffusion coefficients calculated from both light scattering and flow FFF retention were comparable, as well as being consistent with extrapolations from literature values for lower-molecular-mass polyacrylamides.
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