Abstract
This is a well-updated second edition that has been designed and organized for both the novice and experienced cytometrist. The majority of the chapters are aimed primarily for the experimentalist with minimal experience with flow cytometric procedures, although a few discuss relatively advanced topics and specific methodologies such as fluorescence resonance energy transfer (FRET) and fluorescence in situ hybridization analysis by flow cytometry. The introductory chapter by Alice Givan provides a good general description of theoretical and practical aspects, and this overview is well supplemented by a chapter by Carleton and Sigrid Stewart on light and fluorescence scatter displays, gating analyses, and multicolor acquisitions. Several chapters address the analysis of intracellular constituents. A chapter by Perez et al. discusses methods for analyzing phosphoproteins, specifically kinase signaling cascades. Presentation of the different protocols for fixation and permeabilization will be especially helpful to the inexperienced reader. The figures display some of the differences that can be observed, but data in some figures may not be adequately …
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