Abstract
‘Touch’ or trace cell mixtures submitted as evidence are a significant problem for forensic laboratories as they can render resulting genetic profiles difficult or even impossible to interpret. Optical signatures that distinguish epidermal cell populations from different contributors could facilitate the physical separation of mixture components prior to genetic analysis, and potentially the downstream production of single source profiles and/or simplified mixtures. This dataset comprises the results from antibody hybridization surveys using Human Leukocyte Antigen (HLA) and Cytokeratin (CK) probes, as well as surveys of optical properties of deposited cells, including forward scatter (FSC), side scatter (SSC), and fluorescence emissions in the Allophycocyanin (APC) channel. All analyses were performed on “touch” samples deposited by several different contributors on multiple days to assess inter- and intra-contributor variability.
Highlights
Flow cytometry has proven a viable approach for differentiating cell populations in many types of uncompromised forensic mixture sample (Dean et al, 2015; Schoell et al, 1999; Verdon et al, 2015)
Application to ‘touch’ or trace epithelial cell mixtures remains a challenge since many cell surface features are lost or obscured during the process of keratinocyte differentiation, leaving few biochemical or structural features in shed corneocytes that vary between individual contributors
Preliminary research has identified specific optical characteristics—namely, red autofluorescence and forward scatter (FSC) and side scatter (SSC) profiles—that may vary between touch samples deposited by different contributors and collected immediately after deposition (Stanciu et al, 2016)
Summary
Flow cytometry dataset for cells collected from touched surfaces [version 2; peer review: 2 approved].
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