Abstract
This article provides flow cytometry information regarding levels of expression for hematopoietic stem cell markers CD34 and CD133 obtained simultaneously of the bone marrow and peripheral blood from recipients of allogeneic and autologous transplants of PB hematoprogenitors for treating hematological malignancies and who were clinically healthy after ≥100 days following the procedure. CD34 and CD133 expression is compared regarding type of transplant (autologous vs. allogeneic) and sample cell source (bone marrow vs. peripheral blood). Patients were conditioned with a reduced-intensity conditioning regimen. Also shown is the flow cytometry analysis of mononuclear cell and lymphocyte populations in the peripheral blood of both types of recipients, as well as the characterization of immune cells, including T lymphocyte antigenic make up markers CD3, CD4 and CD8, B lymphocytes and NK cells, including total NK, bright and dim subtypes in the peripheral blood of both types of recipients. For further information and discussion regarding interpretation and meaning of post-transplant flow cytometry analysis, please refer to the article “Assessment of immune reconstitution status in recipients of a successful hematopoietic stem cell transplant from peripheral blood after reduced intensity conditioning” [1].
Highlights
Flow cytometry data of bone marrow and peripheral blood of hematology patients who received a stem cell transplant provides a precise assessment of immune status after grafting is complete
This is the first report of the correlation between CD34 þ and CD133þ hematoprogenitors in bone marrow and peripheral blood after hematopoietic grafting
Figure panels depicting the median and interquartile ranges (25,75) of the percentage values of different hematoprogenitor populations expressing the characteristic markers CD34 and CD133 are shown (Fig. 1). To assess their individual contribution to immune cell composition in peripheral blood (PB) according to transplant type, T (Fig. 2) NK (Fig. 3) and B (Fig. 4) lymphocytes were studied
Summary
Flow cytometry data of bone marrow and peripheral blood of hematology patients who received a stem cell transplant provides a precise assessment of immune status after grafting is complete. This is the first report of the correlation between CD34 þ and CD133þ hematoprogenitors in bone marrow and peripheral blood after hematopoietic grafting. Figure panels depicting the median and interquartile ranges (25,75) of the percentage values of different hematoprogenitor populations expressing the characteristic markers CD34 and CD133 are shown (Fig. 1) To assess their individual contribution to immune cell composition in PB according to transplant type, T (Fig. 2) NK (Fig. 3) and B (Fig. 4) lymphocytes were studied. Data depicting the median values and interquartile ranges are presented, comparing the ratio of the different cell components
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