Flow Cytometry-Based Quantification of HIV-Induced T Cell Chemotactic Response.

Abstract

The human immunodeficiency virus (HIV) infects blood CD4 T cells through binding to CD4 and the chemokine co-receptor, CXCR4 or CCR5. This viral binding to CXCR4 or CCR5 also triggers the activation of a variety of signaling molecules such as LIMK/cofilin and WAVE2/Arp2/3 to promote actin dynamics, which are necessary for viral nuclear migration and the latent infection of blood resting CD4 T cells. In this chapter, we describe the methods for quantification of HIV-induced actin polymerization and cofilin phosphorylation in human T cells using flow cytometry.