Abstract
A flow cytometric method was evaluated for performing total leucocyte counts on bovine blood. Fifty blood samples from 19 healthy Holstein cows were analysed on a flow cytometer. The method involved diluting blood with either hypotonic or isotonic saline solution, lysing the red blood cells, and performing a 2-parameter analysis on the basis of cell size and cellular granularity. Leucocyte numbers were determined by creating a window on dot plots of cell size (determined by forward light scatter) vs cellular granularity (determined by the logarithm of side light scatter). Total leucocyte counts determined by flow, cytometer with either diluent were lower (p<0.01) than counts determined by use of an electronic particle counter. The correlations between electronic particle counts and flow cytometric counts using isotonic or hypotonic diluents were 0.857 and 0.458, respectively. The determination of total leucocyte counts using flow cytometry of a blood sample diluted in isotonic saline and treated to lyse red blood cells shows potential as a way for counting leucocytes.
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