Abstract
Epizootiological outbreaks of disseminated neoplasia (DN) have been reported in association with mass mortalities in various bivalve species including the cockle Cerastoderma edule. A flow cytometric (FCM) procedure to study DNA content was successfully adapted and tested in haemolymph cells (haemocytes and neoplastic cells) of the cockle. The FCM results were similar to those obtained by histological analysis (DN diagnosis and haemolymph cell features). FCM analysis revealed differences in DNA content among normal haemocytes (diploid) and neoplastic cells. Four types of cells with abnormal DNA content were found in the haemolymph of affected animals: hypodiploid, hyperdiploid, triploid-sesploid and pentaploid. Our results suggest that the flow cytometric DNA content analysis can be applied to identify neoplastic cell types and to study the association between different cell types and the DN progression or remission in this edible and commercially important bivalve species.
Highlights
Flow cytometric (FCM) analyses have been extensively used in humans, providing valuable information in the diagnosis, classification and prognosis of a variety of diseases (Ward 1999, O’Gorman & Scholl 2002)
Our results suggest that the flow cytometric DNA content analysis can be applied to identify neoplastic cell types and to study the association between different cell types and the disseminated neoplasia (DN) progression or remission in this edible and commercially important bivalve species
Disseminated neoplasia (DN) is a pathological condition that has been reported in association with mass mortalities of various bivalve species (Elston et al 1992), including the cockle Cerastoderma edule (Twomey & Mulcahy 1984)
Summary
Flow cytometric (FCM) analyses have been extensively used in humans, providing valuable information in the diagnosis, classification and prognosis of a variety of diseases (Ward 1999, O’Gorman & Scholl 2002). A flow cytometric (FCM) procedure to study DNA content was successfully adapted and tested in haemolymph cells (haemocytes and neoplastic cells) of the cockle. FCM analysis revealed differences in DNA content among normal haemocytes (diploid) and neoplastic cells.
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