Abstract
We have determined differential scattering cross sections of sphered erythrocytes integrated over the solid angle accepted by our detectors at wavelengths varying between 379.5–632.8 nm, employing Ar+-, Kr+-, and HeNe-laser radiation. Integrated differential cross sections for forward and orthogonal light scatter exhibit a pronounced minimum at about λ = 413 nm, caused by the strong absorption of light by oxyhemoglobin. Experimental data are in good agreement with values calculated by the Mie theory. Simultaneous measurements of forward and orthogonal light scatter using Kr+-laser radiation (λ = 413.1 nm) allow flow cytometric differentiation of (native) erythrocytes, thrombocytes, and leukocytes as well as subpopulations of leukocytes, i.e., granulocytes, lymphocytes, and monocytes in dilute whole-blood samples. Lysis of red blood cells or staining of white blood cells is not required to obtain absolute leukocyte counts or differential white blood cell counts. Cytometry 32:191–197, 1998. © 1998 Wiley-Liss, Inc.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
