Abstract
Non-vaccinated Holstein female calves, 3–31 days old (n=106) were examined in order to determine peripheral blood lymphocyte subpopulations and haematological values. The relative populations of lymphocytes were determined using flow cytometry. Monoclonal antibodies were used to identify BoCD2+, BoCD4+, BoCD8+, B-cells,γ/δ T-cells, and monocytes/neutrophils, respectively. Complete blood counts were also determined. Calves were stratified into four groups by age (days): group A = 3–7 days, group B = 8–14 days, group C = 15–21 days and group D = 22–31 days. Group A calves had 30%–70% lower numbers of all lymphocyte subtypes compared to older calves. Group B calves had significantly lower numbers of B-cells than calves in group D, but were not different from group C. Calves in groups B and C had significantly lowerγ/δ T-cell counts than calves in group D. All other group comparisons of lymphocyte subtypes and the ratios of BoCD4+ to BoCD8+ T-cells were not significantly different.
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