Abstract

Flow cytometry using forward and side light scattering identified three cell subpopulations in the hemolymph of Modiolus kurilensis (Mytilidae). Light microscopic data indicated that they correspond to three hemocyte morphotypes: R1, hemoblasts and agranulocytes (hyalinocytes); R2, semigranulocytes; and R3, granulocytes. The hemoblasts, agranulocytes, and semigranulocytes had a basophilic endoplasm, while the granulocytes were mainly eosinophilic. The proportion of the different cell morphotypes and the level of subpopulation distinction substantially varied among individuals. This seems to be connected with the different functional activities of the hemocytes, depending on the immunological status of the bivalves.

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