Abstract
This chapter discusses the flow cytometric analysis of DNA content in budding yeast. The budding yeast, Saccharomyces cerevisiae, is a popular model organism for the study of eukaryotic cell biology. Saccharomyces cerevisiae was initially an attractive model system for cell cycle research because progression through the cell cycle was mirrored by changes in cell morphology. With advances in cell cycle research, alternative methods for assaying cell cycle progression have become increasingly important. This chapter outlines the methods for the preparation and staining of budding yeast for the analysis of DNA content. It also discusses the machine settings and collection parameters used for the acquisition of data on the flow cytometer. Further, this chapter discusses the interpretation of flow cytometer data, concentrating on points that are unique to the analysis of budding yeast cells.
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