Abstract
<p class="Abstract">In the present study, effects of floroquinone on anaplastic thyroid cancer cell lines and mouse tumor xenografts were investigated. Increase in the concentration of floroquinone from 10 to 100 µM reduced the cell growth from 98 to 17% after 48 hours in HOTHC cells. Similarly, in FRO cells growth rate was found to be 96 and 21%, respectively at 10 and 100 µM concentra-tions of floroquinone. Western blot analysis showed a marked reduction in Bcl-2 expression and increased in Bax, caspase-3 and cleaved PARP expre-ssion in HOTHC cell lines on treatment with floroquinone. Floroquinone treatment of the HOTHC cells led to inhibition of the cobalt chloride-induced increase in the hypoxia-inducible factor 1α (HIF-1α) and vascular endothelial growth factor expression. In HOTHC cells, floroquinone treatment inhibited the tube formation and migration potential significantly compared to control cells. Treatment of the mouse bearing HOTHC tumor xenograft with 50 and 100 mg/kg doses of floroquinone alternatively for one month reduced the tumor volume to 48 and 17%, respectively compared to the control. Thus, floroquinone effectively inhibits growth of thyroid cancer and can be used for its treatment.</p><p><strong>Video Clip</strong>:</p><p><a href="https://www.youtube.com/v/sub0JQlrffc">Migration assay</a>: 1 min 09 sec</p><p> </p>
Highlights
Thyroid cancer, malignant tumor of the endocrine gland, is commonly detected cancer in head and neck with behaviors ranging from occult differentiated to uniformly aggressive and lethal type (Sipos and Mazzaferri, 2010)
HOTHC and FRO anaplastic thyroid cancer (ATC) cell lines were purchased from the Shanghai Institute of Biochemistry and Cellular Biology Chinese Academy of Sciences (China)
The results from MTT assay showed a significant decrease in the growth of HOTHC and FRO ATC cell lines on treatment with floroquinone for 48 hours
Summary
Malignant tumor of the endocrine gland, is commonly detected cancer in head and neck with behaviors ranging from occult differentiated to uniformly aggressive and lethal type (Sipos and Mazzaferri, 2010). Studies have revealed an increase in the rate of detection of thyroid cancer especially in radiologists who are frequently exposed to radiations and work stress (Enewold et al, 2009). Anaplastic thyroid cancer (ATC) is the most lethal with median survival time of 6 months following diagnosis (Catalano et al, 2010; Fassnacht et al, 2009). For the treatment of differentiated thyroid cancers surgery and radioiodine therapy are used, undifferentiated thyroid cancers are resistant to drugs (Urciuoli et al, 2003; Pulcrano et al, 2007; Rajhbeharry singh et al, 2014; Nguyen et al, 2015). The development of novel and efficient treatment therapies are highly needed for these tumors
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