Floral hosts of leaf-cutter bees (Megachilidae) in a biodiversity hotspot revealed by pollen DNA metabarcoding of historic specimens.

South Africa is a megadiverse country with three globally recognised biodiversity hotspots within its borders. Bees in particular show high diversity and endemism in the western part of the country. Not much is currently known about the floral host preferences of indigenous bees in South Africa, with data only available from observational studies. Pollen metabarcoding provides provenance information by utilising DNA analyses instead of floral visitation and traditional microscopic identification to identify pollinator food plants, which can be time consuming and imprecise. In this study, we sampled pollen from leaf-cutter bees (Megachilidae) specimens maintained in a historic insect collection (National Collection of Insects, South Africa) that were originally collected from two florally important areas in South Africa (Succulent Karoo and Savanna) and used metabarcoding to determine pollen provenance. We also sampled pollen from leafcutter bee species with wider distributions, that extend across many different biomes, to determine if these ‘generalist’ species show relaxed floral host specificity in some biomes. Metabarcoding involved sequencing of the nuclear internal transcribed spacer 2 (ITS2) region. Amplicons were compared to a sequence reference database to assign taxonomic classifications to family level. Sequence reads were also clustered to OTUs based on 97% sequence similarity to estimate numbers of plant species visited. We found no significant difference in the mean number of plant taxa visited in the Succulent Karoo and Savanna regions, but the widespread group visited significantly more floral hosts. Bees from the widespread group were also characterised by a significantly different composition in pollen assemblage. The time since specimens were collected did not have an effect on the mean number of taxa visited by any of the bee species studied. This study highlights national history collections as valuable sources of temporal and spatial biodiversity data.

The physiognomically distinct Fynbos and Succulent Karoo Biomes are associated with the winter or all season rainfall region of southwestern South Africa. Fynbos (the more mesic) is co-dominated by small trees, shrubs and reed-like plants. Woody stemmed succulent shrubs, mainly small leaved, dominate the Succulent Karoo. Both biomes contain spectacular floristic richness. Have past climates determined present species richness in this region? We employed GIS-based bioclimatic modelling techniques to address this. After deriving bioclimatic envelopes for both Fynbos Biome (Fynbos Envelope; FYE) and Succulent Karoo (SKE), we used modelled paleoclimate reconstructions to map the distribution of the envelopes at 6, 12 and 18 kBP in the southern and western Cape. At the LGM, FYE possibly had a considerably greater extent along the west coast and the western interior, replacing much of the SKE. At 18 kBP SKE was limited to two separate areas, an extensive plain, the Knersvlakte (South) and montane Richtersveld (North). SKE expanded between 12 and 6 kBP to its present range, and FYE contracted into its current montane distribution. These reconstructions concur with the palynological record of indicator groups of both biomes, with current patterns of relictual Fynbos vegetation, and with Succulent Karoo centres of endemism. Climatic oscillations during the pleistocene probably shifted these biomes iteratively along a North/South axis on the west coast of southern Africa. Mutual vegetation replacements are likely to have accelerated sympatric and allopatric speciation, giving rise to high β and γ species turnover.Key wordsSpecies pumpspeciationpaleoclimatemodellingFynbosSucculent Karoo

The unigeneric tribe Heliophileae encompassing more than 100 Heliophila species is morphologically the most diverse Brassicaceae lineage. The tribe is endemic to southern Africa, confined chiefly to the southwestern South Africa, home of two biodiversity hotspots (Cape Floristic Region and Succulent Karoo). The monospecific Chamira (C. circaeoides), the only crucifer species with persistent cotyledons, is traditionally retrieved as the closest relative of Heliophileae. Our transcriptome analysis revealed a whole-genome duplication (WGD) ∼26.15–29.20 million years ago, presumably preceding the Chamira/Heliophila split. The WGD was then followed by genome-wide diploidization, species radiations, and cladogenesis in Heliophila. The expanded phylogeny based on nuclear ribosomal DNA internal transcribed spacer (ITS) uncovered four major infrageneric clades (A–D) in Heliophila and corroborated the sister relationship between Chamira and Heliophila. Herein, we analyzed how the diploidization process impacted the evolution of repetitive sequences through low-coverage whole-genome sequencing of 15 Heliophila species, representing the four clades, and Chamira. Despite the firmly established infrageneric cladogenesis and different ecological life histories (four perennials vs. 11 annual species), repeatome analysis showed overall comparable evolution of genome sizes (288–484 Mb) and repeat content (25.04–38.90%) across Heliophila species and clades. Among Heliophila species, long terminal repeat (LTR) retrotransposons were the predominant components of the analyzed genomes (11.51–22.42%), whereas tandem repeats had lower abundances (1.03–12.10%). In Chamira, the tandem repeat content (17.92%, 16 diverse tandem repeats) equals the abundance of LTR retrotransposons (16.69%). Among the 108 tandem repeats identified in Heliophila, only 16 repeats were found to be shared among two or more species; no tandem repeats were shared by Chamira and Heliophila genomes. Six “relic” tandem repeats were shared between any two different Heliophila clades by a common descent. Four and six clade-specific repeats shared among clade A and C species, respectively, support the monophyly of these two clades. Three repeats shared by all clade A species corroborate the recent diversification of this clade revealed by plastome-based molecular dating. Phylogenetic analysis based on repeat sequence similarities separated the Heliophila species to three clades [A, C, and (B+D)], mirroring the post-polyploid cladogenesis in Heliophila inferred from rDNA ITS and plastome sequences.

While global patterns of bee diversity have been modelled, our understanding of fine‐scale regional patterns is more limited, particularly for under‐sampled regions such as Africa. South Africa is among the exceptions on the African continent; its bee fauna (ca. 1253 species) has been well collected and documented, including mass digitising of its natural history collections. It is a region with high floral diversity, high habitat heterogeneity and variable rainfall seasonality. Here, we combine a South African bee species distributional database (877 bee species) with a geospatial modelling approach to determine fine‐scale (~11 × 11 km grid cell resolution) hotspots of bee species richness, endemism and range‐restricted species. Our analyses, based on the probabilities of occurrence surfaces for each species across 108,803 two‐minute grid cells, reveal three bee hotspots of richness: Winter rainfall, Aseasonal rainfall and Early‐to‐late summer rainfall. These hotspots contain large numbers of endemic and geographically restricted taxa. Hotspots with particularly high bee diversity include the Fynbos, Succulent Karoo and Desert biomes; the latter showing 6–20 times more species per unit area than other biomes. Our results conform with global species‐area patterns: areas of higher‐than‐expected bee density are largely concentrated in Mediterranean and arid habitats. This study further enhances our knowledge in identifying regional and global hotspots of richness and endemism for a keystone group of insects and enabling these to be accounted for when setting conservation priorities.

The governability of small-scale fisheries located adjacent to Marine Protected Areas (MPAs) in South Africa has increasingly come under scrutiny as communities, social science researchers, NGOs and human rights activists challenge current governance approaches that disregard the socio-cultural rights and livelihood needs of fishing communities living within or adjacent to MPAs. Drawing on research conducted in seven case studies in South Africa, this chapter explores the current mismatch between the realities facing fishing communities impacted by MPAs and the state-centric and natural science-based approach to governance adopted by South Africa’s fisheries management and conservation authorities. This approach to MPA governance persists despite a suite of policy reforms and political rhetoric that indicates the embrace of a more people-centred approach to natural resource governance. The key focus of this chapter is to gain a deeper understanding as to why this mismatch persists despite almost 20 years of democracy and policy reforms. While the devastating impact of South Africa’s political history is evident in all cases, other factors that inhibit meaningful change and formation of robust governance systems, are highlighted. These include the persistence of a natural-science paradigm; the divergent principles, values, worldviews and images amongst governance actors; institutional shortcomings; failure to recognize and respect local and customary forms of governance; and the lack of attention to implementation mechanisms that are informed by all governance actors.

Psammotermes allocerus Silvestri, 1908 is the only described species representing the genus Psammotermes Desneux, 1902 in Southern Africa. The large geographical range of this subterranean termite covers both summer and winter rainfall regimes. Deadwood is the preferred food when available, but in more arid habitats, both live and dead grasses form the major dietary component. Along the Namib Desert margins, the species’ localised herbivory creates circular bare patches known as fairy circles. For a regional phylogeographic study of this species, we sampled 65 sand termite populations within drier parts of Namibia, South Africa, and Angola. Based on combined molecular and ecological data, we found considerable genetic diversification within P. allocerus. Analyses of two mitochondrial markers (COI, COII), including a Bayesian inference tree, haplotype analysis and genetic distances suggest a delineation into seven highly differentiated genetic groups. The ‘Succulent Karoo’ group is additionally characterised by unique features of the royal chamber, nest and tunnel system. In conclusion, our data suggest that P. allocerus should be not regarded as one species but as a species complex. Termites of each analysed group ‘Northern Namib’, ‘Western Kalahari Basin’, ‘Nama’, ‘Southwestern Kalahari’, ‘East Gariep’, ‘Southern Namib’ and ‘Succulent Karoo’ should be considered as distinct species. The species name P. allocerus should be used for termites of the ‘Succulent Karoo’.

Genetic diversity, acaricide resistance status and evolutionary potential of a Rhipicephalus microplus population from a disease-controlled cattle farming area in South Africa

The Bombyliidae genera Enica Macquart, 1834 and Nomalonia Rondani, 1863, restricted to South Africa, are synonymized. Currently, one species of Enica is known from South Africa, Enica longirostris (Wiedemann, 1819), and six Nomalonia species are newly combined with Enica: Enica clavicornis (Hesse, 1956), comb. nov.; Enica eremophila, (Hesse, 1975), comb. nov.; Enica henicoides (Hesse, 1956), comb. nov.; Enica imitata (Hesse, 1956), comb. nov.; Enica sporanthera (Hesse, 1956), comb. nov.; and Enica syrticola (Hesse, 1956), comb. nov. A new species, Enica adelphesp. nov. from the Richtersveld in the Northern Cape of South Africa, is described. All Enica species are redescribed and a dichotomous key with photographic references is provided for ease of identification. Specimen occurrence data and photographs are provided for each species. Each species of Enica occurs in at least one of three biodiversity hotspots in South Africa, i.e., Succulent Karoo, Cape Floristic Region, and Maputaland-Pondoland-Albany, with E. longirostris occurring in all three.

lIgIntroduction.l/Ig Traditional birth attendants (TBAs) are still frequently utilised in rural areas in South Africa, even when mothers have access to formal health care facilities. Studies reveal that utilisation of TBAs can be beneficial in some contexts, with support and supervision from the Western health sector. lbrglIgAim.l/Ig To evaluate a training programme for TBAs on HIV / AIDS and safe delivery. lbrglIgMethod.l/Ig The study used a pre-post training evaluation design of 50 TBAs in two primary health care clinic areas in rural South Africa. lbrglIgResults.l/Ig Most TBAs had some knowledge of risk signs during pregnancy. At follow-up assessment HIV / AIDS knowledge had significantly increased and HIV risk practices when assisting during a delivery had significantly decreased. Most TBAs were involved in HIV/sexually transmitted infection (STI) management such as risk assessment, risk reduction counselling, distribution of condoms, community education and home-based care. After the training significantly more TBAs conducted prenatal check-ups, assessed the baby's position in the uterus and took the mother's and baby's pulse, and fewer TBAs conducted abnormal or complicated deliveries. lbrglIgConclusion.l/Ig Training of TBAs can increase their knowledge, improve their attitudes and reduce risk practices. TBAs need skilled and equipped available support to carry out basic preventive measures in the obstetric patient, anticipate and identify obstetric complications, administer nevirapine prophylaxis, and make appropriate and timely referrals backed up with efficient referral mechanisms to reduce maternal morbidity and mortality.

The purpose of this paper is to determine whether there is a difference in the development of shopping lists and use of advertisements as pre-store food-buying practices in terms of planned shopping by South African consumers who dwell in different socio-economic status (SES) areas. The paper also considers the influence of shopper and socio-demographic characteristics on pre-store food-buying practices in a developing country. A self-administered questionnaire was used to survey 1 200 consumers in retail stores in low, middle and high SES areas in South Africa. A generalised linear model was employed for the statistical analysis of pre-store food-buying practices within the SES area groups in a developing country. South African consumers that reside in high SES area displayed the largest of shopping list development, while consumers who dwell in low SES areas showed the highest incidence of advertisement usage. Several shopper and socio-demographic characteristics were also found to have an influence on pre-store food-buying practices in different SES areas in South Africa. A qualitative approach would offer a deeper understanding of consumers' pre-store food shopping predispositions as opposed to the quantitative approach, which was adopted for this study. A longitudinal design would also provide a more extensive representation of pre-store food shopping practices over a longer time frame than cross-sectional research. The survey was conducted on Saturdays, whereas consumers who shop during the week may have different shopping and socio-demographic characteristics. Astute food brands, marketers and grocery stores could use the findings of this study to assist with their marketing efforts that they direct at consumers in different SES areas in South Africa and other developing countries. The findings of this study may assist consumers in developing countries, especially those who reside in low SES areas, with food-buying strategies to reduce food costs, make wiser purchase decisions and reduce shopping. No study (to the best of the researchers' knowledge) has considered shopping list development and use of advertisements' pre-store food-buying practices in different SES areas in a developing country. Furthermore, there is a dearth of research analysing shopper and socio-demographic characteristics in relation to pre-store food-buying practices among different SES areas in developing and developed countries.

This study investigated the impact of parental financial socialisation on financial attitude of young black African adults in rural and low-income area in South Africa through quantitative research approach, survey design and self-administered questionnaire. Data was collected from young black African adults in Fetakgomo Tubatse and Intsika Yethu local municipalities, low-income and rural areas in South Africa. Multiple regression analysis was used to test the relationship between parental financial behaviour, parental financial monitoring, parental financial discussion, parental financial communication, and parental financial teaching with financial attitude. The results showed that parental financial communication and parental financial teaching had a significant positive relationship with financial attitude. While parental financial behaviour, parental financial monitoring and parental financial discussion had no significant positive relationship with financial attitude. Therefore, the overall results indicated that parental financial socialisation has no impact on financial attitude of young black African adults in rural and low-income area in South Africa. This study provided recommendations to parents, young black African adults, financial educators, financial services institutions, financial counsellors and the government.

This study investigated the impact of parental financial socialisation on financial decision-making of young black African adults in rural and low-income area in South Africa. Data was collected through self-administered questionnaire from 423 young black African adults in Fetakgomo Tubatse and Intsika Yethu local municipalities, low-income and rural areas in South Africa. Multiple regression analysis was used to test the relationship between parental financial behaviour, parental financial monitoring, parental financial discussion, parental financial communication, and parental financial teaching with financial decision-making. The results indicated that parental financial communication had a significant positive relationship with financial decision-making. While parental financial behaviour, parental financial monitoring, parental financial discussion, and parental financial teaching had no positive relationship with financial decision-making. Therefore, the overall results indicated that parental financial socialisation has no impact on financial decision-making of young black African adults in rural and low-income area in South Africa. This study provided recommendations to parents, young black African adults, financial educators, financial services institutions, and financial counsellors.

The geographical distribution and habitats of three liver fluke intermediate hosts in South - Africa and the health implications involved

African Vernacular Symbols of Black Intersex Children in Sinethemba Ngubane's Installations (2007-2016)

Pollinators, even floral generalists (=polyleges), typically specialize during individual foraging bouts, infrequently switching between floral hosts. Such transient floral constancy restricts pollen flow, and thereby gene flow, to conspecific flowers in mixed plant communities. Where incipient flowering species meet, however, weak cross-fertility and often similar floral traits can yield mixed reproductive outcomes among pollinator-dependent species. In these cases, floral constancy by polyleges sometimes serves as an ethological mating barrier. More often, their foraging infidelities instead facilitate host introgression and hybridization. Many other bee species are oligolectic (taxonomic specialists for pollen). Oligoleges could be more discriminating connoisseurs than polyleges when foraging among their limited set of related floral hosts. If true, greater foraging constancy might ensue, contributing to positive assortative mating and disruptive selection, thereby facilitating speciation among their interfertile floral hosts. To test this Connoisseur Hypothesis, nesting females of two species of oligolectic Osmia bees were presented with randomized mixed arrays of flowers of two sympatric species of their pollen host, Balsamorhiza, a genus known for hybridization. In a closely spaced grid, the females of both species preferred the larger flowered B. macrophylla, evidence for discrimination. However, both species' females showed no floral constancy whatsoever during their individual foraging bouts, switching randomly between species proportional to their floral preference. In a wider spaced array in which the bouquets reflected natural plant spacing, foraging oligolectic bees often transferred pollen surrogates (fluorescent powders) both between conspecific flowers (geitonogamy and xenogamy) and between the two Balsamorhiza species. The Connoisseur Hypothesis was therefore rejected. Foraging infidelity by these oligolectic Osmia bees will contribute to introgression and hybridization where interfertile species of Balsamorhiza meet and flower together. A literature review reveals that other plant genera whose species hybridize also attract numerous oligolectic bees, providing independent opportunities to test the generality of this conclusion.