Abstract
Poly(γ-glutamic acid) (γ-PGA) prepared from a culture of Bacillus subtilis had a high flocculating activity and its flocculation properties were investigated. Flocculating activity of γ-PGA in a kaolin suspension was stimulated by the addition of Ca 2+, Mg 2+ or Fe 2+ to the suspension. High flocculating activity of γ-PGA was induced in a kaolin suspension by addition of Fe 3+ or Al 3+ and pH adjustment to the neutral range. Various inorganic suspensions of active carbon, acid clay, solid soil, and calcium and magnesium compounds were flocculated by γ-PGA. Flocculation of organic suspensions such as cellulose and yeast by γ-PGA occurred when Fe 2+, Fe 3+ or Al 3+ were added and the pHs of the suspensions were adjusted to the neutral range. From these flocculation properties in various suspensions, it may be possible to use γ-PGA as a new biodegradable, harmless biopolymer flocculant.
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