Abstract
The flocculation of cell, cell debris and soluble proteins from 2-keto- L-gulonic acid process media with six different charge densities of methacryloyloxyethyl trimethylammonium chloride—acrylonitrile (DMMC-AN) copolymers as flocculant was studied to investigate the influence of charge density on flocculation. It was found that the effectiveness of flocculation and optimal flocculation dosages depend on the cationic density of the flocculant. With optimal cationic density, DMMC-AN copolymer can remove 97% of the cell debris and 95% soluble proteins by 30 min sedimentation under gravity. The optimal flocculation dosage varies between 500 and 700 ppm. The study also showed that DMMC-AN copolymer has no selectivity on removal of soluble proteins in the spent medium, since the soluble proteins with different sizes can be removed efficiently by the test of Sephadex G-150 column chromatography, which contributed to the purification of the non-protein biological products solution.
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