Abstract
A flexible enzymatic acetylcholinesterase biosensor based on an electrolyte-gated carbon nanotube field effect transistor is demonstrated. The enzyme immobilization is done on a planar gold gate electrode using 3-mercapto propionic acid as the linker molecule. The sensor showed good sensing capability as a sensor for the neurotransmitter acetylcholine, with a sensitivity of 5.7 μA/decade, and demonstrated excellent specificity when tested against interfering analytes present in the body. As the flexible sensor is supposed to suffer mechanical deformations, the endurance of the sensor was measured by putting it under extensive mechanical stress. The enzymatic activity was inhibited by more than 70% when the phosphate-buffered saline (PBS) buffer was spiked with 5 mg/mL malathion (an organophosphate) solution. The biosensor was successfully challenged with tap water and strawberry juice, demonstrating its usefulness as an analytical tool for organophosphate detection.
Highlights
Organophosphorus pesticides (OPs) are widely used in agriculture, and are potentially toxic for human health
We report a flexible spray-deposited carbon nanotube field effect transistors (CNTFETs)-based enzymatic sensor for the detection of organophosphorus pesticide
Chamber was mounted around the active area of the CNTFETs to serve as a compartment for 60 μL of the analyte solution, which was exchanged manually using a Gilson pipette
Summary
Organophosphorus pesticides (OPs) are widely used in agriculture, and are potentially toxic for human health. Organophosphorus pesticides produce toxicity by inhibiting the enzyme acetylcholinesterase (AChE), which is responsible for the removal of the neurotransmitter acetylcholine (ACh) from the synaptic cleft through hydrolysis [2]. OPs bind to AChE and produce a stable complex. In this process, the serine residue of AChE is blocked, causing a drastic accumulation of ACh at the nerve synapse, interfering with the normal nervous system function [3]. The serine residue of AChE is blocked, causing a drastic accumulation of ACh at the nerve synapse, interfering with the normal nervous system function [3] This results in rapid twitching of voluntary muscles, followed by paralysis. The traditional techniques for the detection of OPs are based on analytical methods such as spectrophotometry [4], spectrofluorimetry [5], infrared spectroscopy [6], photothermal [7], chemiluminescence [8], quartz crystal microbalance [9], mass
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
