Abstract
BackgroundAlthough electrical stimulation of the peripheral and central nervous systems has attracted much attention owing to its potential therapeutic effects on neuropsychiatric diseases, its non-cell-type-specific activation characteristics may hinder its wide clinical application. Unlike electrical methodologies, optogenetics has more recently been applied as a cell-specific approach for precise modulation of neural functions in vivo, for instance on the vagus nerve. The commonly used implantable optical waveguides are silica optical fibers, which for brain optogenetic stimulation (BOS) are usually fixed on the skull bone. However, due to the huge mismatch of mechanical properties between the stiff optical implants and deformable vagal tissues, vagus nerve optogenetic stimulation (VNOS) in free-behaving animals continues to be a great challenge.ResultsTo resolve this issue, we developed a simplified method for the fabrication of flexible and stretchable polymer optical fibers (POFs), which show significantly improved characteristics for in vivo optogenetic applications, specifically a low Young’s modulus, high stretchability, improved biocompatibility, and long-term stability. We implanted the POFs into the primary motor cortex of C57 mice after the expression of CaMKIIα-ChR2-mCherry detected frequency-dependent neuronal activity and the behavioral changes during light delivery. The viability of POFs as implantable waveguides for VNOS was verified by the increased firing rate of the fast-spiking GABAergic interneurons recorded in the left vagus nerve of VGAT-ChR2 transgenic mice. Furthermore, VNOS was carried out in free-moving rodents via chronically implanted POFs, and an inhibitory influence on the cardiac system and an anxiolytic effect on behaviors was shown.ConclusionOur results demonstrate the feasibility and advantages of the use of POFs in chronic optogenetic modulations in both of the central and peripheral nervous systems, providing new information for the development of novel therapeutic strategies for the treatment of neuropsychiatric disorders.
Highlights
Electrical stimulation of the peripheral and central nervous systems has attracted much attention owing to its potential therapeutic effects on neuropsychiatric diseases, its non-cell-type-specific activation characteristics may hinder its wide clinical application
We developed a simplified method for the fabrication of a flexible and stretchable core/clad structured PDMS/hydrogel polymer optical fiber (POF) and investigated the feasibility of its optogenetic applications in vivo
The PDMS core of the POF was fabricated via a thermal drawing process, and a hydrogel film was applied as a cladding layer
Summary
Electrical stimulation of the peripheral and central nervous systems has attracted much attention owing to its potential therapeutic effects on neuropsychiatric diseases, its non-cell-type-specific activation characteristics may hinder its wide clinical application. To precisely manipulate the activities of specific neurons or neural circuits in particular brain regions, implantable SOFs are usually fixed on the skull bone to illuminate the lightsensitive protein expressed cells [22,23,24]. This strategy can provide a relatively stable interface between the optical implants and brain tissues that usually undergo negligible (or low) deformations in vivo, which has been applied in dissecting the complex neural processing mechanisms of neuropsychiatric diseases and behavioralrelated phenomena at the functional level. Chronic optogenetic stimulation, especially vagus nerve optogenetic stimulation (VNOS), in free-behaving animals is still a great challenging task
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