Flexible adhesive GelMA/SF-based patch embedded with polydopamine N-halamine nanoparticles for repair of infected wounds

Abstract

Complex bacterial infection and inevitable movement are the two most concerning issues in joint wound healing. In this work, N-halamine structure was introduced into polydopamine nanoparticles (PDNPs) and encapsulated in a gelatin methacryloyl (GelMA)/silk fibroin (SF) matrix to endow the patches with efficient and broad-spectrum antibacterial activity. Zone of inhibition and in vivo antibacterial tests confirm that the addition of N-halamine containing organic nanoparticles as antibacterial components is a convenient and feasible strategy in the development of wound healing antibacterial materials. Here, dopamine methacrylamide (DMA) was synthesized and grafted onto the gelatin methacryloyl in patches with UV irradiation to get a reasonable adhesion. The liver prick injury model showed that the flexible adhesive GelMA/SF-based patch could be fixed on the wound area with rapid hemostatic effect. Furthermore, the resultant patches possess good flexibility, breathability and excellent biocompatibility. The histology analysis indicated the designed patches demonstrated less inflammatory response, significantly enhanced vascularization and the regeneration of dermis and epidermis. In summary, these N-halamine hemostatic GelMA/SF patches hold a promise for diverse practical acute trauma treatments.