Abstract
Flax enzymatic retting with composite enzyme produced by microbes with inexpensive substrates is widely researched due to less contamination and lower cost. Bacillus licheniformis HDYM-04, isolated from a liquid sample of flax retting pool, efficiently produced degumming enzymes after 48 h of fermentation with inexpensive konjaku flour, consisted of 587.5 U/mL pectinase, 365.2 U/mL mannanase, and 140.1 U/mL xylanase. Almost half the maximum activity of three above-mentioned degumming enzymes was maintained at pH 4.0–6.0 which demonstrated its stability in pH condition of flax retting. After 120 h of retting with this composite enzyme, scanning electronic microscopy showed more significant reduction in gummy components on the fiber surface than those of water retting. The fiber strength was 182.4 ± 9.3 N, 14.3% higher than water-retted samples. The long fiber rate and fiber yield also verified higher fiber productivity. The results permitted this degumming composite enzyme an applicable potential in flax retting.
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