Abstract
Novel bioactive components have greatly attracted attention as they demonstrate health benefits. Reversed-phase high performance liquid chromatography (RP-HPLC) showed that isoflavonoid compounds of kudzu root (Pueraria lobata) fermented by Eurtotium cristatum and extracted using de-ionized water were higher active compared with non-fermented. A model of H2O2-inducd cell damage was built using rat pheochromocytoma line 12 (PC12) cell to observe the protective effect of non-fermented kudzu root (Pueraria lobata) (NFK) and fermented kudzu root (Pueraria lobata) (FK). Cell viability and apoptosis were analyzed through inverted microscopy and flow cytometry. The level of lactate dehydrogenase, catalase activity, superoxide dismutase, glutathione, and reactive oxygen species (ROS) were evaluated. Results showed that NFK and FK could significantly protect PC12 cell against damage caused by H2O2-induced oxidative stress. The intracellular antioxidant system was increased, protected the cell membrane inhibit H2O2-induced apoptosis by scavenging of ROS. Moreover, NFK and FK regulated the cell cycle to prevent cell apoptosis. Isoflavonoid from the kudzu root especially fermented kudzu root with E. cristatum are potentially therapeutic drugs against diseases induced by oxidative damage.
Highlights
Health foods and their by-products have recently attracted considerable attention
Fan et al [10] reported that apoptosis of neuronal cell contributes to the apoptotic pathway, and such contribution is caused by mitochondrial homeostasis, which is affected by major reactive oxygen species (ROS)
This study examined whether E. cristatum fermented kudzu root (Pueraria lobata) exert neuro-protective effects against H2O2-induced ROS production and whether fermented kudzu root inhibits apoptosis of pheochromocytoma line 12 (PC12) cell
Summary
Health foods and their by-products have recently attracted considerable attention. Various phytochemical components have been recognized as natural antioxidants, which are used to counteract reactive oxygen species. Fan et al [10] reported that apoptosis of neuronal cell contributes to the apoptotic pathway, and such contribution is caused by mitochondrial homeostasis, which is affected by major reactive oxygen species (ROS) (e.g., superoxide radical and hydroxyl radical, by energy production, and mediate mitochondrial-dependent pathway). Cells can protect themselves from damages, injury, and apoptosis induced by oxidative stress through their internal antioxidant defense mechanisms [10]. In PC12 cells, H2O2 peroxide can induce intracellular reactive oxygen species (ROS), thereby motivating cell oxidative damage, resulting in the expression of related genes or related apoptotic proteases, resulting in cell apoptosis. This study examined whether E. cristatum fermented kudzu root (Pueraria lobata) exert neuro-protective effects against H2O2-induced ROS production and whether fermented kudzu root inhibits apoptosis of PC12 cell
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