Abstract
BackgroundThe Tridax procumbens flavonoids (TPF), are well known for their medicinal properties among local natives. The TPF are traditionally used for dropsy, anaemia, arthritis, gout, asthma, ulcer, piles, and urinary problems. It also used in treating gastric problems, body pain, and rheumatic pains of joints. The TPF have been reported to increase osteogenic functioning in mesenchymal stem cells. However, their effects on osteoclastogenesis remain unclear. The TPF isolated from T. procumbens and investigated the effects of the TPF inhibit on osteoclast differentiation and bone resorption activities using primary osteoclastic cells. Osteoclast formation was assessed by counting the number of tartrate resistant acid phosphatase (TRAP) positive multinucleated cells and by measuring both TRAP activities.ResultsThe TPF significantly suppressed the RANKL-induced differentiation of osteoclasts and the formation of pits in primary osteoclastic cells. The TPF also decreased the expression of mRNAs related to osteoclast differentiation, including Trap, Cathepsin K, Mmp-9, and Mmp-13 in primary osteoclastic cells. The treatment of primary osteoclastic cells with the TPF decreased Cathepsin K, Mmp-9, and Mmp-13 proteins expression in primary osteoclastic cells.ConclusionThese results indicated that TPF inhibit osteoclastogenesis and pits formation activities. Our results suggest that the TPF could be a potential anti-bone resorptic agent to treat patients with bone loss-associated diseases such as osteoporosis.
Highlights
The Tridax procumbens flavonoids (TPF), are well known for their medicinal properties among local natives
Effect of the TPF on osteoclast formation To examine the effect of the TPF on osteoclast formation, cultures of the primary osteoclast cells were treated with the TPF at concentrations of 0, 50 and 100 μg/ml for 7 days
The present study found that the TPF at doses between 50 and 100 μg/ml significantly inhibited receptor activator of NF-kB ligand (RANKL)-induced differentiation of osteoclasts in the primary osteoclastic cells
Summary
The Tridax procumbens flavonoids (TPF), are well known for their medicinal properties among local natives. Osteoclast formation was assessed by counting the number of tartrate resistant acid phosphatase (TRAP) positive multinucleated cells and by measuring both TRAP activities. Recent attention has been paid to biologically active extracts and compounds from plant species used in herbal medicines [9] It has been proved effective in the treatment of diseases simultaneously mitigating many of the side effects which are often associated with synthetic antibiotics [10]. The TPF from leaves and root bark are traditionally used for dropsy, anaemia, arthritis, and gout It is used for the treatment of asthma, ulcer, piles, and urinary problems [12]. Present results demonstrated that the TPF inhibited osteoclasts differentiation and TRAP activities
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