Flavonoid production by T. lactinea: screening of culture conditions via OFAT and optimization using response surface methodology (RSM)

Abstract

The culture conditions for flavonoid production by the fungus Trametes lactinea was screened using one-factor-at-a-time (OFAT) and optimized with response surface methodology. T. Lactinea was found to grow well in Medium 3, and the highest flavonoid was produced in Medium 2. Medium 2 contains yeast extract, glucose, KH2PO4, Na2HPO4 · 12H2O, MgSO4 · 7H2O, and (NH4)2 · SO4, whereas Medium 3 contains yeast extract, glucose and peptone. Four selected parameters in OFAT were further optimized for flavonoid production by T. lactinea using response surface methodology via Box Behnken design (BBD). Interactions of culture conditions and optimization of the system were then studied using BBD with four levels of the four variables in a batch flask culture. Experimentation showed that the model developed based on BBD had predicted flavonoid production with R2=0.9983. The predicted flavonoid production was optimum (20.02 μg/mL) when the culture conditions were at 8.1 days of incubation period, 5.2 mL of inoculum, incubation temperature at 34.6°C, and the production medium with initial pH 6. The results indicated that BBD method was effective in optimizing the culture conditions of flavonoid production by T.lactinea.