Flavonoid isolation and identification of mother-in-law’s tongue leaves (sansevieria trifasciata) and the inhibitory activities to xanthine oxidase enzyme

Abdullah Abdullah,Heri Hermansyah,Sri Ningsih,Tania Surya Utami,Angelina Angelina,Mauhibah Yumna,Rita Arbianti,E Kusrini,E.A Setiawan,A Yatim,F.H Juwono

doi:10.1051/e3sconf/20186703011

Abstract

Hyperuricemia is a disease that is characterized by high uric acid levels, in which the number of victim increase year by year in the worldwide. Flavonoid is an active compound with inhibitory activity towards Xanthine Oxidase Enzyme which is a compound that plays a role in the formation of uric acid in the body.Sansevieria trifasciatais an ornamental plant which is also useful as a source of antibacterial and antioxidant agent. Studies of S.trifasciataas Xanthine Oxidase Enzyme inhibitor have not been reported. This research isolate flavonoid compounds using open column chromatography from crude extract of S.trifasciataleaves that extracted by sonication method. There are six eluent used to isolate flavonoid which are methanol : ethyl acetate, chloroform : ethyl acetate, chloroform : ethyl acetate : methanol. Wilstater test is used to select the fraction that rich of flavonoid. The best result from isolation step that contains flavonoid is assessed the inhibitory activity of xanthine oxidase. It is analyzed qualitative using Liquid Chromatography Mass Spectrometry (LCMS). The inhibition percentage showed that fraction 3 was potential to inhibit XO by 85.48 %. LC-MS chromatogram can show that crude extract and positive fraction of isolation were containing falvonoid.

Highlights

Hyperuricemia, associated with gout, results from the overproduction or underexcretion of uric acid and it is greatly influenced by a high dietary intake of foods rich in nucleic acids
The Best eluent was determined with Thin Layer chromatography (TLC) silica G 60 F254 using different eluent that used to isolate flavonoid which are methanol:ethyl acetate (4:1), chloroform:ethyl acetate (1:6, 9:1 and 1:2), chloroform:ethyl acetate:methanol (1:2:1 and 1:2:2)
2.2.4 Isolation Methode plant remedies used for gout by examining their xanthine oxidase inhibitory activity and whether a relationship exists between enzyme inhibition and the flavonoid and other content

Summary

Introduction

Hyperuricemia, associated with gout, results from the overproduction or underexcretion of uric acid and it is greatly influenced by a high dietary intake of foods rich in nucleic acids. The traditional diets of Indonesia were especially high in wild meat, which could be expected to contribute to gout, meats (especially organ meats), leguminous seed, some types of seafood and food yeasts that are considered rich of nucleic acids [1]. The catalysis of xanthine by the enzyme xanthine oxidase (XO) (EC 1.2.3.2) can lead to the accumulation of uric acid and causing gout. Many plants are used by Indigenous people for the treatment of gout, or diseases with associated symptomologies such as rheumatism or arthritis, and we postulate that these may contain XO inhibitors. XO inhibitors have been found in a wide variety of plants used in traditional herbal medicines for the treatment of gout and rheumatism for example Chile [3] Paraguay [4] [5] [6] and Panama [7]

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