Abstract
Tetracera indica Merr. in Musi Banyuasin, is one of the traditional medicine used by the community for the treatment of kidney stone disease and gout, but this claim is not recorded in the treatment of kidney stones and gout in Indonesia. In this study, isolation of antioxidant compound from ethyl acetate extracts of supit (Tetracera indica) was done. The isolation was carried out through step gradient polarity extraction, and separated and purified by chromatography technique. The determination of the structure of the isolated compound was performed by spectroscopy method including UV, IR, and NMR 1D and 2D, and antoxidant activity was determined by DPPH method. An active antioxidant compound was isolated from ethyl acetate extract in form of yellow solid (15 mg). Based on spectroscopic analysis the isolated compound was 5,8-dihydroxy-7-methoxyflavone. The compound showed strong antioxidant activity (IC50 8.25 μg/mL) higher than standard ascorbic acid (IC50 11.3 μg/mL). This data concluded that efficacy of supit (Tetracera indica) for the treatment related to antioxidant activity (uric acid) is proven by the identification of one antioxidant compound of this plant.
Highlights
Indonesia is a country with the second largest diversity of plants in the world, but there are still many medicinal plants that have not been fully utilized (Hanafi, Nina, Zorni, and Nurbaiti, 2005)
The powder Tetracera indica of 550 g was extracted with n-hexane solvent, ethyl acetate and ethanol respectively and after concentrated was obtained n-hexane extract 250 mg, ethyl acetate 3,50 g and ethanol extract 3.7 g
Antioxidant activity of isolated compound Antioxidant activity test was done by DPPH method with concentration variation 100, 50, 25, 12.5 and 6.75 ppm
Summary
Indonesia is a country with the second largest diversity of plants in the world, but there are still many medicinal plants that have not been fully utilized (Hanafi, Nina, Zorni, and Nurbaiti, 2005). In vitro antioxidant activity test of 9 selected plant extracts is one of them is supit stems, showed high antioxidant activity with value of % inhibition 69.6% at test concentration 1000 ppm. In this paper will be reported antioxidant compound active by DPPH method from ethyl acetate extract stem of supit.
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