Flavisolibacter carri sp. nov., isolated from an automotive air-conditioning system.

Abstract

A Gram-stain negative, aerobic, non-motile, rod-shaped and yellow bacterium, designated TX0651T, was isolated from an automotive air-conditioning system. Phylogenetically, the strain groups with the members of the genus Flavisolibacter and exhibits high 16S rRNA gene sequence similarities with Flavisolibacter ginsenosidimutans Gsoil 636T (97.4%), Flavisolibacter ginsengiterrae Gsoil 492T (96.3%) and Flavisolibacter ginsengisoli Gsoil 643T (96.2%). DNA-DNA relatedness between TX0651T and F. ginsenosidimutans KCTC 22818T and F. ginsengiterrae KCTC 12656T were determined to be less than 40%. The low levels of DNA-DNA relatedness identifies the strain TX0651T as a novel species in the genus Flavisolibacter. The major cellular fatty acids were identified as iso-C15:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), iso-C15:1 G and iso-C17:0 3-OH. The predominant respiratory quinone was identified as MK-7. The polar lipids were found to be comprised of phosphatidylethanolamine, unidentified amino-glycophospholipids, an unidentified aminophospholipid, an unidentified amino lipid and unidentified lipids. The DNA G+C content of the strain was determined to be 31.2mol%. On the basis of the phenotypic, genotypic and chemotaxonomic characteristics, strain TX0651T should be classified in a novel species in the genus Flavisolibacter, for which the name Flavisolibacter carri sp. nov. (= KACC 19014T = KCTC 52836T = NBRC 111784T) is proposed.