Abstract
Flatpea ( Lathyrus sylvestris L.) tissues originating from several European accessions were regeneration potential on several media formulations. Low-frequency, whole-plant regeneration of flatpea was obtained from hypocotyl-derived callus cultures, although the primary response of all accessions was root formation without shoots. Conditions for optimum whole plant-regeneration included callus initiation on a modified Schenk-Hildebrandt low-ammonium (SH-LA) medium containing 5.0 μM indoleacetic acid (IAA) and 2.5 μM zeatin followed by two subcultures and transfer of calli to morphogenesis medium, basal (SH-LA) medium containing 1.0 μM zeatin and 0.1 μM IAA. A medium containing 2.5 μM zeatin and 1.0 μM IAA was also effective for morphogenesis. Root and shoot primordia developed in several cultures, approximately 60 days after explant transfer to callus induction medium. 2,4-Diaminobutyric acid (DABA) content was not appreciably reduced in regenerated material.
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