Abstract
The formation of reactive species and free radicals in water soluble elastin hydrolysates have been investigated by pulse radiolysis and flash photolysis. Elastin hydrolysates were obtained by hydrolysis of elastin extracted from aorta.An investigation of the photochemical properties of elastin hydrolysates in water was carried out using nanosecond laser irradiation. The transient spectra of elastin hydrolysates solution excited at 266nm showed two bands. One of them with maximum at 295nm and the second one with maximum at 400nm.The reactions of hydrated electrons and OH radicals with elastin have been studied by pulse radiolysis. In the absorption spectra of products resulting from the reaction of elastin with eaq- small maximum absorption in UV and visible light was observed. In the absorption spectra of products resulting from the reaction of the hydroxyl radicals with elastin two bands were observed. The first one at 320nm and the second one at 410nm. Reaction of OH radicals with elastin hydrolysates lead to formation of Tyr phenoxyl radicals with absorption at 410nm. The influence of the addition of sodium azide NaN3 on the formation of the transients was evaluated.
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