Abstract

Biochemical oxygen demand (BOD) is a widely used index of water quality in wastewater treatment; however, conventional measurement methods are time-consuming. In this study, we analyzed a novel flame-oxidized stainless steel anode (FO-SSA) for use as the probe of bioelectrochemical system (BES)-based biosensors to monitor the BOD of treated swine wastewater. A thinner biofilm formed on the FO-SSA compared with that on a common carbon-cloth anode (CCA). The FO-SSA was superior to the CCA in terms of rapid sensing; the response time of the FO-SSA to obtain the value of R2 > 0.8 was 1 h, whereas the CCA required 4 h. These results indicate that the FO-SSA offers better performance than traditional CCAs in BES biosensors and can be used to improve biomonitoring of wastewater.

Highlights

  • Biochemical oxygen demand (BOD) is a widely used index for measuring the amount of biodegradable organic matter in wastewater

  • BOD biosensors based on microbial fuel cells (MFCs) have emerged as an alternative to the conventional method [1]

  • Kim et al [2] reported on a mediator-free MFC-based BOD biosensor for the first time

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Summary

Introduction

Biochemical oxygen demand (BOD) is a widely used index for measuring the amount of biodegradable organic matter in wastewater. Current generation in MFC-based biosensors depends on the anode reaction, and on the cathode reaction, and changes in cathode conditions can potentially affect current generation in a BOD-independent manner. To circumvent the cathode dependency, we recently developed a novel bioelectrochemical system (BES)-based BOD biosensor equipped with a potentiostat [5]. The anodic potential of the biosensor is kept constant by the potentiostat; the current generation is expected to depend on only the anode reaction based on the potential control. Potentiostat; the current generation is expected to depend on only the anode reaction based on the potential control. AAnew newmethod method oxidizes the surface of a stainless steel(SSA). Thatthat oxidizes the surface of a stainless steel anode using fire reportedly reportedlythe improves current output.

Biosensor
Biosensor Operation and Analysis
Results
Efficacy of the FO-SSA as a BES-Based BOD Biosensor Probe
Potential Applications of FO-SSA-Equipped BOD Biosensors
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