Flame ionization detector responses to ethyl esters of sand eel (Ammodytes lancea) fish oil compared for different gas and supercritical fluid chromatographic systems

Abstract

A fish oil fatty acid ethyl ester mixture derived from the sand eel (Ammodytes lancea) has been analysed using various capillary gas chromatography (GC) and supercritical fluid chromatography (SFC) methods and 39 components including cholesterol have been identified. The results of the analytical SFC and GC experiments are compared showing a good reproducibility within methods and a fair agreement between methods. The advantages and disadvantages of the employment of manual and autosampler split injection, splitless injection, and cold on-column injection in GC as well as the use of polar and non-polar columns in GC and SFC are discussed.