Abstract
BackgroundThe cause and pathophysiology of ulcerative colitis are both mainly unknown. We have previously used whole-genome microarray technique on biopsies obtained from patients with ulcerative colitis to identifiy 5 changed mucosal transcripts. The aim of this study was to compare mucosal expressions of these five transcripts in ulcerative colitis patients vs. controls, along with the transcript expression in relation to the clinical ulcerative colitis status.MethodsColonic mucosal specimens from rectum and caecum were taken at ambulatory colonoscopy from ulcerative colitis patients (n = 49) with defined inflammatory activity and disease extension, and from controls (n = 67) without inflammatory bowel disease. The five mucosal transcripts aldolase B, elafin, MST-1, simNIPhom and SLC6A14 were analyzed using quantitative real-time PCR.ResultsSignificant transcript differences in the rectal mucosa for all five transcripts were demonstrated in ulcerative colitis patients compared to controls. The grade of transcript expression was related to the clinical disease activity.ConclusionThe five gene transcripts were changed in patients with ulcerative colitis, and were related to the disease activity. The known biological function of some of the transcripts may contribute to the inflammatory features and indicate a possible role of microbes in ulcerative colitis. The findings may also contribute to our pathophysiological understanding of ulcerative colitis.
Highlights
The cause and pathophysiology of ulcerative colitis are both mainly unknown
The use of microarray technique analyses on mucosal specimens obtained from both patients with established Ulcerative colitis (UC) and controls has allowed identification of candidate genes, which are valuable in research on UC pathogenesis
In the present study the transcripts selected are based on our earlier individual whole-genome microarray screening and quantitative real-time PCR (RT-PCR) in patients with UC [14], where five changed genes/transcripts were identified; aldolase B, elafin, MST-1, simNIPhom, and SLC6A14
Summary
The cause and pathophysiology of ulcerative colitis are both mainly unknown. We have previously used whole-genome microarray technique on biopsies obtained from patients with ulcerative colitis to identifiy 5 changed mucosal transcripts. Ulcerative colitis (UC) is a disorder characterized by chronic mucosal inflammation of the large intestine. It is frequently associated with various extraintestinal manifestations. The use of microarray technique analyses on mucosal specimens obtained from both patients with established UC and controls has allowed identification of candidate genes, which are valuable in research on UC pathogenesis. These UC candidate genes must be carefully selected, since recent evaluations of microarray data have revealed considerable divergence after examination of similar tissues [11,12,13]. The pathophysiological properties of SimNIPhom have not yet been clarified, but the other transcript products have potential importance in secretion [15,16], anti-microbiological activity [17], and cell-mediated immune response [18]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
