Abstract
SEQUENCING A GENOME IS just the first step in understanding the biology of an organism. Proteins really do the work. With that in mind, two groups independendy report similar methods for discovering protein complexes within yeast. Both groups use proteins to fish out protein interactions. Yeast proteins are turned into bait by attaching a tag that allows them to be captured in an irnmunoaffinity purification. The captured complexes are then separated by gel electrophoresis, and excised gel spots are analyzed by mass spectrometry (MS). A team of scientists from MDS Proteomics in Toronto and Odense, Denmark; Mount Sinai Hospital inToronto; and the University of Toronto used the method to detect 3,617 protein interactions, starting with 725 bait proteins [Nature, 415,180 (2002)}. The other team, led by Giulio Superti-Furga, vice president of biology at Cellzome AG and team leader at the European Molecular Biology Laboratory both in Heidelberg, Germany identified 232 distinct multiprotein complexes ...
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