Fish peptone development using enzymatic hydrolysis of silver carp by-products as a nitrogen source in Staphylococcus aureus media.

Meysam Fallah,Somayeh Bahram,Seyed Roholla Javadian

doi:10.1002/fsn3.198

Abstract

Fish peptone was produced using enzymatic hydrolysis of silver carp filleting by-products by alcalase and trypsin. Also, the efficiency of the hydrolysates as a nitrogen source in Staphylococcus aureus medium was compared with commercial TSB. The results indicated that the protein hydrolysate from alcalase and trypsin had high protein content (92.92%, 91.53 respectively), and degree of hydrolysis (4.94%, 4.6% respectively).The results showed that silver carp filleting waste can be an efficient source for fish peptone production as a nitrogen source for S. aureus medium. However, the type of the used proteolytic enzyme considerably affected the performance of the resulting peptone despite the same DH. Fish peptone produced by alcalese performed significantly (P < 0.05) better than commercial TSB as a media for the bacteria while the performance of the trypsin peptone was not as good as the commercial medium.

Highlights

Seafood processing industry generates up to 60% byproducts including head, skin, trimmings, fins, frames, viscera and roes, and only 40% fish products for human consumption (Dekkers et al 2011)
Higher degree of hydrolyzed (DH) observed for hydrolysates produced by alcalase compared to trypsin was reported by Ovissipour et al (2009) who compared the effect of four different enzymes in the hydrolysis of Persian sturgeon viscera
The application of different hydrolysis time caused the production of protein hydrolysates with approximately equal DH for both enzymes

Summary

Introduction

Seafood processing industry generates up to 60% byproducts including head, skin, trimmings, fins, frames, viscera and roes, and only 40% fish products for human consumption (Dekkers et al 2011). Waste and by-products discarded by fisheries are currently increasing, driven by both a net increase in seafood consumption and the changing consumer trend toward ready-to-eat products. These large quantities of fish byproducts require appropriate management, especially because they create serious pollution and disposal problems in both developed and developing countries. On other hand, these by-products are considered to be protein-rich material that is normally processed into low market-value products, such as animal feed, fish meal, and fertilizer (Hsu 2010). One of the efficient biotechniques that are currently employed is enzymatic hydrolysis of fish proteins that allows the production of a broad spectrum of food ingredients or industrial products for a wide range of applications, including fish peptones (Chalamaiah et al 2012)

Methods

Results

Conclusion