Abstract
BackgroundHemorrhagic shock followed by resuscitation stimulates an inflammatory response. This study tests the hypothesis that treatment with fish oil will attenuate inflammatory responses and stabilize hemodynamics.MethodsMale SD rats (n = 48; 250~300 g) were randomly divided into 4 groups: SHAM, hemorrhagic shock (HS), hemorrhagic shock/resuscitation (HS/R) and fish oil (FO). Shock was induced, and a mean arterial pressure (MAP) was maintained at 35 to 40 mmHg for 60 minutes. Resuscitation was carried out by returning half of the shed blood and Ringer's lactate solution to the animal. In FO group, fish oil (0.2 g/Kg) was infused through caudal vena at 30 minutes after shock. Half of each group was killed at 30 minutes and at 4 hours after resuscitation. Then several kinds of inflammation and oxidative stress indicators such as IL-6, MPO and GSH were tested.ResultFO group required less resuscitative fluid and had higher urinary output at the recovery periods from hemorrhagic shock than HS/R group(p < 0.001). After resuscitation, the MAP of HS/R group markedly declined than FO group (p < 0.001). The inflammatory indexes of FO group were lower than HS group and HS/R group and the same as sham group. But the level of endotoxin in FO group was significantly higher than sham group at 4 hours.ConclusionFish oil pretreatment before fluid resuscitation showed a beneficial effect to the hemodynamic stabilization and inflammation reduction in HS/R rat model.
Highlights
Hemorrhagic shock (HS) remains a major cause of death in both civilian and combat traumatic injury [1]. It followed by resuscitation is considered as an insult frequently induces a systemic inflammatory response syndrome (SIRS) that results in multiple-organ dysfunction syndrome (MODS) [2], which is a major clinical problem
mean arterial pressure (MAP) in the shock group, resuscitation group, and fish oil (FO) group fell to the same level during shock
The decline was found in the two groups, but FO group had a smaller decrease than the hemorrhagic shock/resuscitation (HS/R) groups did
Summary
Hemorrhagic shock (HS) remains a major cause of death in both civilian and combat traumatic injury [1]. It followed by resuscitation is considered as an insult frequently induces a systemic inflammatory response syndrome (SIRS) that results in multiple-organ dysfunction syndrome (MODS) [2], which is a major clinical problem. Our hypothesis was that infusion of fish oil before resuscitation in hemorrhagic shock rats, would help to minimize the post-resuscitation inflammatory responses and to stabilize hemodynamics. Hemorrhagic shock followed by resuscitation stimulates an inflammatory response. This study tests the hypothesis that treatment with fish oil will attenuate inflammatory responses and stabilize hemodynamics
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