Abstract
We present here the physical mapping of the 5S rDNA locus in six wild and five cultivated taxa of Capsicum by means of a genus-specific FISH probe. In all taxa, a single 5S locus per haploid genome that persistently mapped onto the short arm of a unique metacentric chromosome pair at intercalar position, was found. 5S FISH signals of almost the same size and brightness intensity were observed in all the analyzed taxa. This is the first cytological characterization of the 5S in wild taxa of Capsicum by using a genus-derived probe, and the most exhaustive and comprehensive in the chili peppers up to now. The information provided here will aid the cytomolecular characterization of pepper germplasm to evaluate variability and can be instrumental to integrate physical, genetic and genomic maps already generated in the genus.
Highlights
Capsicum L., or chili peppers, (Solanaceae) is a New Word genus distributed from Mexico to Argentina that comprises 31 species, several of which are of considerable economic importance
The information reported here, broadly expands the limited knowledge of 5S rDNA mapping in Capsicum, but will be undoubtedly useful to integrate physical, genetic and genomic maps already generated in the genus (Tanksley et al 1988, Kang et al 1997, Wu et al 2009, Qin et al 2014, Kim et al 2014)
Additional features associated to the chromosome that carries the 5S rDNA are provided in Table I, i.e. the chromosome length (CL), the centromeric index (CI) and the relative chromosome length (RCL)
Summary
Capsicum L., or chili peppers, (Solanaceae) is a New Word genus distributed from Mexico to Argentina that comprises 31 species, several of which are of considerable economic importance. Previous fluorescent in situ hybridization (FISH) assays revealed the loci number and localization of the 5S rRNA gene in only a few Capsicum species, by means of different probes, some of them derived from unrelated taxa (Park et al 1999, 2000, Scaldaferro et al 2006, Kwon and Kim 2009). More comprehensive approaches led to the development of several pepper maps (Wu et al 2009 and references therein) and recently, to the genome sequencing of wild and cultivated species of chili peppers (Kim et al 2014, Qin et al 2014). Despite 45S rRNA genes have been mapped onto a pepper genetic linkage map (Wu et al 2009), information regarding 5S rDNA genes in Capsicum, is still lacking
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