Fish Identification based on Partial Fragments of The Mitochondrial COI Subunit I Gene

Tuah Nanda Merlia Wulandari

doi:10.1051/e3sconf/202132201038

Abstract

The mtDNA sequences revealed that several of the fish studied were Hampala macrolepidota and Barbonymus gonionotus. The objective of this research was to learn the pattern of COI gene in mtDNA and establish a phylogenetic tree. Basic Local Alignment Search Tool-nucleotide (BLASTn) confirmed that Barbonymus gonionotus froma the Ranau Lake, South Sumatera has 100% matching ranges to the species from Memberamo River (Indonesia), India, Bangladesh, Thailand (Mae Khlong), Indo-Myanmar, and Malaysia_1. The lowest closeness (98.76%) is related to species from Thailand (Lower Ing). The Blast investigation appears us that the level of familiarity was very high, it is coming to 98-100% in Barbonymus gonionotus. Hampala macrolepidota had 100% matching ranges to the species from Indonesia (SouthaSumatera_1) and Vietnam. They had 99.05%-99.84% closeness from Malaysia_1,2&3, Indonesia (South Sumatera_2&3, Java and Bali_1,2&3).

Highlights

Based on water volume, Ranau Lake is the second-largest the largest lake in Indonesia [15]
Basic Local Alignment Search Tool-nucleotide (BLASTn) evaluation confirmed that Barbonymus gonionotus from Lake Ranau in South Sumatra showed a 100% similarity level to the identical species from Memberamo River (Indonesia), India, Bangladesh, Thailand (Mae Khlong), Indo-Myanmar, Malaysia_1
The stage of similarity acquired from BLASTn analysis was very high, attaining 98100 % in Barbonymus gonionotus (Table 1)

Summary

Introduction

Ranau Lake is the second-largest the largest lake in Indonesia [15]. Some species that can be found in Ranau Lake are Hampala macrolepidota and Barbonymus gonionotus. Both belong to the family of cyprinidae. COI-based on molecular method can identify specimens precisely, reliably, accessible, and at low cost [11]. Barbonymus gonionotus is very suitable as an ornamental fish The population of this type of fish has experienced a decline in the last ten years due to logging, overfishing, and fishing practices that are not environmentally friendly by fishermen in the waters of Nagan [1]. The study aims to identify several types of fishes using DNA sequences in the Ranau Lake and to obtain a more definite identification based on information on the nucleotide sequences of Barbonymus gonionotus and Hampala macrolepidota

Objectives

Results

Conclusion