Abstract
A procedure for using fish cells in culture as an additional parameter in the study of aquatic toxicants is described, and a comparison is made between the dose response of the fathead minnow and fathead minnow cells in culture, to zinc intoxication. The cells were found to be more sensitive than were the whole animals. The calculated safe concentration, based on the whole animal studies, was found to reduce the mitotic index of the cell cultures by approxately 50 per cent. A concentration of zinc representing one-tenth of the 96 hr TLm of the fathead minnows was found to have no effect on the cell cultures. Using these tissue culture criteria it is concluded that one-tenth of the 96 hr TLm is a demonstrably safer concentration for determining maximum permissible concentrations than is the calculated biologically safe concentration.
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