Abstract
BackgroundB-cell chronic lymphocytic leukemia (B-CLL) is the most common leukemia in the Western world. Major progress has been made in assessing typical chromosomal abnormalities and recognition of the correlation of these chromosomal abnormalities with laboratory features and clinical course of the disease. The most frequent genomic changes are deletions at 13q14, 11q22-23 and 17p13 and trisomy of chromosome 12.ObjectivesThe aim of this study was to investigate the frequency of chromosomal aberrations in B-CLL patients’ peripheral blood and/or bone marrow using a molecular cytogenetic method, interphase fluorescence in situ hybridization (I-FISH) and to evaluate the correlation between these genomic changes and clinical findings.Patients and MethodsI-FISH analyses were performed on bone marrow and blood samples of 66 B-CLL patients.ResultsDeletion of 17p13 was found in 11 (16.6%) and deletion 6q21 was present in 5 (7.5%). Statistical analyses were performed to investigate the correlation of these molecular-cytogenetic findings with family history, Rai staging and CD38 marker. No clear differences in distribution was noted for del17p13 and del6q21 among patients with and without family history, and no direct correlation was noted between these genomic changes and CD38 marker, but the correlation of del17p13 and Rai stage was significant. There was a high frequency of Rai stage II within del17p13 patients.ConclusionsIt was demonstrated that the presence of del6q21 in B-CLL patients indicates poor prognosis and on the contrary, presence of del17p13 points at the good prognostic value of the disease.
Highlights
B-cell chronic lymphocytic leukemia (B-CLL) is the most common leukemia in the Western world
B-cell chronic lymphocytic leukemia (B-CLL) is usually described as the most common leukemia in the United States, Canada, and Western Europe, whereas it is rare in Japan and infrequent in other Asian societies [1]
The aims of this study were first to verify the frequency of del (6q21) and del (17p13) in B-CLL patients by conventional cytogenetic methods and FISH technique, and second to determine the correlation between these two abnormalities and prognostic factors, including Rai staging, CD38 marker and family history
Summary
B-cell chronic lymphocytic leukemia (B-CLL) is the most common leukemia in the Western world. Objectives: The aim of this study was to investigate the frequency of chromosomal aberrations in B-CLL patients’ peripheral blood and/or bone marrow using a molecular cytogenetic method, interphase fluorescence in situ hybridization (I-FISH) and to evaluate the correlation between these genomic changes and clinical findings. Statistical analyses were performed to investigate the correlation of these molecular-cytogenetic findings with family history, Rai staging and CD38 marker. The reported maleto-female sex ratio is about 1.5–2:1, which means B-CLL is more common in men than women [3]. It is a common cancer not even in the Western countries, and in Iran [4]. Considering the high frequency of del17p13 observed within patients in this study, we recommend that cytogenetic evaluation of del17p13 be performed for patients routinely
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