Year-end Sale % OFF 
Abstract
pBSSB1 is a 27 kb non-bacteriophage-related linear plasmid first found in Salmonella enterica serovar Typhi (S. Typhi), but the mechanism underlying the replication of pBSSB1 is currently unknown. Previous reports showed that the factor for inversion stimulation (Fis) encoded by fis can affect the replication, transcription and other processes through binding DNA. Here, a fis deletion mutant of S. Typhi (Δfis) was prepared through the homologous recombination mediated by suicide plasmid and the loss of pBSSB1 in Δfis was observed surprisingly by pulsed field gel electrophoresis (PFGE). Subsequently, the loss of pBSSB1 was verified by PCR and Southern blot. In addition, the motility of Δfis was deficient and the flagellin of Δfis could not be detected by 2-dimensional polyacrylamide gel electrophoresis. All these results show that Fis is essential for the stability of pBSSB1 and affects the motility of S. Typhi.
Highlights
The factor for inversion stimulation (Fis) encoded by fis is a small DNA-bending nucleotide-associated protein which plays a role in the transcriptional regulation of a number of genes in diverse bacterial species [1]
Typhi (Dfis) through the homologous recombination mediated by suicide plasmid, and performed pulsed field gel electrophoresis (PFGE) to investigate the genome structure of Dfis
Linear plasmids are relatively common in bacterial species such as Streptomyces and Borrelia, pBSSB1 is the first nonbacteriophage-related linear plasmid to be described in the Enterobacteriaceae that contains no detectable homology sequence of bacteriophage [8]
Summary
The factor for inversion stimulation (Fis) encoded by fis is a small DNA-bending nucleotide-associated protein which plays a role in the transcriptional regulation of a number of genes in diverse bacterial species [1]. DnaA operon is composed of dnaA, dnaN and recF, coding for the DnaA protein which can recognize the origin of replication oriC, b subunit of DNA polymerase III which is responsible for the extension of the newly-replicated DNA chains and the RecF protein which is involved in the recombination and the repair of DNA, respectively [12,13] In light of these functions of Fis, we hypothesized that Fis may play a very important role in the replication of pBSSB1. Only the complementary strain Dfis(pBADfis) can host this linear plasmid while the Dfis and Dfis(pBAD) cannot These results suggest that fis is essential for the stability of plasmid pBSSB1. The results show that the motility of Dfis was deficient and the flagellin of Dfis could not be detected
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
